When you need stuff to work… they don’t!

What a stupid week!!! I know it’s only Monday, but has been a kind of weird couple of weeks. It’s almost Easter break, which means I get to go home for a few days and then I’ll be travelling to the US for 10 days, so I should be ecstatic, right? I am, don’t get me wrong, I never needed to get home so bad as I do now! But being away for roughly 3 weeks, means I have to leave a lot of things done, mainly projects that are not directly related to my PhD thesis.

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So, a merely 9 days left of my work schedule until May, I desperately stuff to go right, to work, to not get contaminated. I need my extractions to work, my PCR’s to work, I need that the blancs turns out, well, clean. I need the equipment to work properly and to not run out of lab supplies or extremely important solutions and reagents. So, obviously, all of this goes exactly the opposite way…

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The extractions work well(ish), but then the blancs emit some signals that shouldn’t be there, and that probably aren’t… because the some primers decided to act out! How I love to have a contaminated primer solution!!! Then there are my sex typing primers that decided not to type anything really… Obviously by now the only thing that was actually working fine was the real time PCR, until today, when someone (stupid motherf*****r kid decided to steal the ancient laptop that ran the machine). I’m not even going to mention the fact that I have to sequence stuff before Thursday, because of the break, and now I won’t be able to because of some stupid person!!!! OMG, I’m so damn mad right now!

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