When you need stuff to work… they don’t!

What a stupid week!!! I know it’s only Monday, but has been a kind of weird couple of weeks. It’s almost Easter break, which means I get to go home for a few days and then I’ll be travelling to the US for 10 days, so I should be ecstatic, right? I am, don’t get me wrong, I never needed to get home so bad as I do now! But being away for roughly 3 weeks, means I have to leave a lot of things done, mainly projects that are not directly related to my PhD thesis.

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So, a merely 9 days left of my work schedule until May, I desperately stuff to go right, to work, to not get contaminated. I need my extractions to work, my PCR’s to work, I need that the blancs turns out, well, clean. I need the equipment to work properly and to not run out of lab supplies or extremely important solutions and reagents. So, obviously, all of this goes exactly the opposite way…

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The extractions work well(ish), but then the blancs emit some signals that shouldn’t be there, and that probably aren’t… because the some primers decided to act out! How I love to have a contaminated primer solution!!! Then there are my sex typing primers that decided not to type anything really… Obviously by now the only thing that was actually working fine was the real time PCR, until today, when someone (stupid motherf*****r kid decided to steal the ancient laptop that ran the machine). I’m not even going to mention the fact that I have to sequence stuff before Thursday, because of the break, and now I won’t be able to because of some stupid person!!!! OMG, I’m so damn mad right now!

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Working all weekend long

I’m in a kind of weird type of research, where my experiments don’t take exactly too long, but somedays, if my PCR at 10 a.m. does not work, I can’t do it again in the same day, and I get nothing left to do in the lab… this implies that there are days that my day is done by 1 p.m., and that everyday of the week counts, which means, working holidays and weekdays.

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This was this kind of weekend. My DNA extractions take a grand total of 3 days (with 2 overnight incubations), which meant all weekend alone in the lab.

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Don’t get me wrong, I love coming to work on weekends, I get much more done than on weekdays, because every piece of equipment is available and there are no waiting lines for anything… and I get to hear loud music!

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There is just one problem with being alone in the lab: I’m alone, so this is pretty much my reaction each time I hear any kind of sound…

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Lab stresses!!!

I found this really cool blog on Tumblr, THATS NOT HOW YOU PIPETTE, about the wrongful use of pipettes on TV. It’s hilarious!!!!!

I found it on a day that I was particularly stressed out about tips (pipette tips), why? Well, I don’t have OCD or anything of the sorts, but this…

… Just stressed me out! Why on Earth would you not follow some kind of logic when taking the tips out of the box? WHY?! Why would you not do it somehow like this?!

Well, I will leave you with some of the hilarious posts about pipettes on TV, from the blog I mentioned above.

By the way CSI, this is not the right way to use a centrifuge…

It hurts my hear a little to see all this great equipment and material being misused…